Equipment (Shared Laboratory)

The aim of the RDN is the strengthening of the existing excellent neuroscience research by funding state-of-the-art group equipment. Thus, in the last years the RDN purchased joint equipment for a variety of interdisciplinary research projects. These instruments are available for all members of the department. You can find information about contact details in the list below.


Magnetic resonance imaging

7 Tesla magnetic resonance imaging system

The MRI-scanner is especially configured for MRI-studies of small mammals such as rodents and pigeons. Detailed pictures of brain structures in high contrast can be obtained based on the 7 tesla magnetic field strength. This high resolution is for example very important for experiments that aim at covering physiological to cognitive topics.
The scanner is funded by the Stiftung Mercator.

Contact:
Prof. Dr. O. Güntürkün or
Prof. Dr. D. Manahan-Vaughan

Location:
Scanner Centre, building NI/NT



3Tesla magnetic resonance imaging system (Humans & Primates)

Multimodal stimulation system for functional MRI

The multimodal stimulation system for functional MRI consists of a video system to produce visual stimuli and video sequences, which are presented via stereo-LCD-glasses. The communication system for the presentation of acoustical stimulation patterns is especially adapted for the use in a MRI-scanner. The whole stimulation system is available for investigations in the 3 Tesla-scanner and therefore it is hosted at the Neurological Clinic, Bergmannsheil.

Contact:
Prof. Dr. M. Tegenthoff

Location: University Clinic Bergmannsheil



Electrophysiology

Electroencephalography (EEG)-lab

Multi electrode array system

Patch-Clamp Setup

Microscopy

Dual-color STED (STimulated Emission Depletion) microscope

The STED microscope is based on the setup published by Bückers et al. (Optics Express 2011, 19(4), p.3130. DOI: 10.1364/OE.19.00313032011)

The light source of this setup is a
Fianium ALP super-continuum laser offering a super-continuum as well as two beams for STED at 711nm and 745nm. The STED microscope is integrated into a commercial, automated epifluorescent microscope (iMIC, Till Photonics, today FEI Munich) which allows taking overview images. The filter sets currently available for the epifluorescent microscope allow recording the following dyes:
Atto 590, Abberior Star 580, Alexa 594
Atto 647N, Abberior Star 635P, Abberior Star Red, Alexa 647
GFP, FITC and similar

Objectives for the epifluorescent overview mode are Olympus 4× and 20× long working distance objectives.
The epifluorescent microscope is built in a heating chamber that allows temperature control and controlling the CO2-level of the atmosphere, allowing for live cell recordings. STED imaging is possible with one orange-red and a second far-red dye (Details can be found in the dye selection How-to) .

Objective for STED recording is an Olympus APON60XOTIRF and confocal pinholes are 75µm in diameter, however, pinholes can be changed. The microscope is equipped with a Yanus IV beam scanner (Till Photonics, today FEI munich).

Contact:
Dr. P. Happel

Location:
RUBION, building NI 06/135



2-Photonen confocale microscope

Confocale multiphoton laser scanning microscope

Neurolucida system

The Neurolucida system consists of a software/hardware package for performing brain mapping, neuron tracing and reconstruction in 2 D and 3D.

Contact:
Prof. Dr. K. Funke

Location: Medical Faculty, Dep. Neurophysiology, MA 4/159



DNA Sequencing

Affymetrix DNA Microarray Scanner

This system is used for microarray-based DNA sequencing approaches. You have the flexibility to run mapping, resequencing, gene expression and targeted genotyping applications using one platform.

Contact:
Dr. W. Gerding

Location:: Medical Faculty, Dep. of Human Genetics, MA 5